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DIRECT COOMBS IgG / C3 (DAT)

Department: Blood Bank
Test Synonym(s): DAT, Coombs, Direct Coombs Test (DCT)
Test Components: IgG (DAG); C3 (DAC)
CPT Codes: 86880 for each
Methodology: Serological
Tube Type: Lavender top tube (EDTA)
Specimen: Blood, plasma
Alternate Specimen: None
Pediatric Requirements: 2 mL
Volume: 2 mL
Temperature: Room temperature
Stability: 3 days
Reasons for Rejection: Excessive hemolysis
Days Performed: Daily
Times Performed: Continuous
TAT: 1 day
Special Instructions: Specimen must be labeled with patient's first and last name, Medical Record number (non VUMC: unique identifier), collection date, and phlebotomist ID. PPID must be performed. Collection time on requisition if not captured in VUMC EMR
Click here for Specimen Labeling Policy.


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