HIV 1/2 Ab Conf Assay (HIB)
Department: Virology
Test Synonym(s): LAB864, HIB, HIV Ab Differentiation Assay, HIV Confirmation
Test Components: HIV-2 envelope peptides gp36 and gp140, HIV-1 polymerase peptide gp31, HIV-1 envelope recombinant protein gp160, HIV-1 core recombinant protein gp24, HIV-1 envelope peptides (Group M and O) gp41
CPT Codes: 86701, 86702
Methodology: Immunochromatographic
Reference Range: HIV antibodies negative
Tube Type: Red top (no gel)
Specimen: Serum
Alternate Specimen: Plasma (EDTA, Sodium Citrate, Sodium Heparin)
Pediatric Requirements: Red or lavender microtainer (1mL) x2
Volume: 1 tube
Minimum Volume: Red or lavender microtainer (1mL) x2
Temperature: Refrigerated (2-8 C)
Stability: Labile specimen; keep refrigerated
Reasons for Rejection: Obvious microbial contamination; specimens other than serum or plasma; heat-inactivated specimens; specimens that have incurred more than 5 freeze-thaw cycles
Days Performed: Daily
Times Performed: Monday - Friday: 6:30 am to 11:00 pm. Saturday and Sunday: 7:00 am to 3:30 pm
TAT: Same day - 2 days
Significance: The HIV-1/ HIV-2 Antibody Differentiation test discriminates between HIV-1 and HIV-2 antibodies. Results for each type are reported. A negative result indicates absence of detectable HIV antibodies; however, a negative test result does not preclude infection with HIV.
Special Instructions: A recommendation to order HIV-1 nucleic-acid testing on a separate specimen will be made for certain results. This multi-test algorithm for the diagnosis of HIV is endorsed by the Centers for Disease Control and Prevention and Association of Public Health Laboratories. The HIV-1/HIV-2 Antibody Differentiation test is not approved for use to screen blood, plasma, cell, or tissue donors.
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