HIV P24 Ag and HIV 1/2 Ab (HIV)

Department: Virology
Test Synonym(s): LAB159, HIV, HIV ag/ab combo, HIV P24 Antigen and HIV 1/2 Antibody
Test Components: HIV-1 p24 antigen, HIV-1/HIV-2 antibodies
CPT Codes: 86703 ab, 87390 ag
Methodology: Chemiluminescence
Reference Range: Nonreactive
Tube Type: Red top (no gel)
Specimen: Serum
Alternate Specimen: Lavender top (EDTA) plasma
Pediatric Requirements: Red or lavender microtainer (1 mL) x 2
Volume: 1 tube
Minimum Volume: Red or lavender microtainer (1 mL) x 2
Temperature: Allow to clot, store refrigerated (2-8 degrees C)
Stability: Labile specimen. Keep refrigerated.
Reasons for Rejection: Same assay within 4 days, grossly hemolyzed, obvious microbial contamination, fluids other than serum/plasma
Days Performed: Monday - Friday
Times Performed: Morning
TAT: Same day - 2 1/2 days
Significance: Negative result does not rule out infection with HIV; patient may be seronegative during acute infection.
Special Instructions: HIV-1 p24 antigen and antibodies to HIV-1&2 are detected simultaneously in this assay. This assay does not distinguish positive signals generated from viral antigen and antibody. Repeatedly reactive specimens are confirmed by HIV-1/HIV-2 differentiation assay. A negative result does not rule out infection with HIV; the patient may be negative during seroconversion. The performance of this assay has not been established for individuals younger than 2 years old.

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