Vitamin D Total (TVD)
Department: Chemistry
Test Synonym(s): LAB3418, TVD, 25 - Hydroxy - Vitamin D, Total Vitamin D, Vitamin D, 25-OH, Total
CPT Codes: 82306
Methodology: Chemiluminescent Microparticle Immunoassay (CMIA)
Reference Range: Vitamin D status: Deficiency <10; Insufficiency 10-24; Sufficiency 25-80; Toxicity >80
Tube Type: Gold top SST (Clot activator with gel) - 5 mL
Specimen: Serum
Alternate Specimen: Red Top (no gel)
Pediatric Requirements: Note: Pediatric reference intervals have not been established for this test
Volume: 2 mL Serum
Minimum Volume: 1 Gold top
Temperature: Separate within one hour and store at 2-8C until delivery
Stability: Room temperature: 3 days; Refrigerated: 6 days; Frozen: 3 months
Reasons for Rejection: Gross hemolysis or lipemia, improper collection, QNS
Days Performed: Daily
Times Performed: Continuous
TAT: 1 day
Significance: This test is appropriate for screening for 25-OH vitamin D status, diagnosis of vitamin D deficiency or toxicity, and/or monitoring of Vitamin D supplementation, <10 ng/mL (severe deficiency), 10-24 ng/mL (mild to moderate deficiency), 25-80 ng/mL (optimum levels), >80 ng/mL (toxicity possible).
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