MDL MPL Exon 10 Mut Anly ()
Department: Molecular Diagnostics
Test Synonym(s): LAB6146, MPL Exon 10 Mutation Analysis, MDL MPL
Test Components: MPL Exon 10
CPT Codes: 81339
Methodology: PCR coupled with Sanger Sequencing and Capillary Electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Blood, bone marrow, purified DNA - 1 micron (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers blood or bone marrow
Volume: 1 tube
Minimum Volume: 2 microtainers blood or bone marrow; purified DNA - 100 ng
Temperature: EDTA: Room temperature or refrigerated, purified DNA: refrigerate or freeze.
Stability: EDTA tube - Room temperature: 2 days, Refrigerated: 7 days; Purified DNA - indefinitely at -70C
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Variable
Times Performed: Variable
TAT: 14 days
Significance: Identification of a pathogenic variant in exon 10 of the MPL gene can aid in the diagnosis of a myeloproliferative neoplasm.
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
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