MIDL PCR Monkeypox ()
Department: Molecular Infectious Disease
Test Synonym(s): LAB6422, MPX, Pox, Non-variola, Poxvirus, Orthopox, Orthopoxvirus
CPT Codes: 87593
Methodology: Real-Time PCR
Reference Range: Not detected
Tube Type: Universal Transport Media
Specimen: Submit swab of lesion. The following lesion locations are acceptable - skin, oral, genial and rectal
Pediatric Requirements: No additional requirements
Volume: 3 mL
Minimum Volume: 1 ml
Temperature: Refrigerated: 1 week; Frozen: >1 week
Stability: Refrigerated: 1 week; Frozen: >1 week
Reasons for Rejection: Leaking samples; Alternative specimen sent without Medical Director approval; Improper storage
Days Performed: Monday, Wednesday and Friday
Times Performed: One time a day
TAT: 60 hours
Significance: This assay detects multiple zoonotic non-variola orthopoxviruses, including monkeypox virus. Monkeypox virus is currently the only circulating orthopoxvirus. Results must be interpreted in the clinical context. A positive result can be interpreted as indicating monkeypox infection in the appropriate clinical context.
Special Instructions: Vesicles contain the highest amount of Monkeypox Virus. Submit swab of lesion. The following lesion locations are acceptable - skin, oral, genital and rectal. Swab multiple sites with individual swabs. Ensure appropriate PPE is worn when collecting specimens.
PLEASE NOTE: Currently this test is limited to VUMC Provider orders only.
For more information, please contact the lab at 615-875-5227.
Click here for information from the CDC web site.
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