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Flow Fetal Cell (HbF) Assay (FCF)

Department: Hematopathology/Flow Cytometry
Test Synonym(s): LAB3461, FCF, Fetal Cell Stain, FRBC Detection Assay, Kleihauer-Betke
Test Components: %Fetal Cells Detected; Recommended RhIG Dose
CPT Codes: 85460
Methodology: Flow Cytometry
Tube Type: Lavender top (EDTA)
Specimen: Whole Blood
Alternate Specimen: None
Pediatric Requirements: 2 mL Whole Blood
Volume: 5 mL Whole Blood
Minimum Volume: 2 mL Whole Blood
Temperature: Room temperature
Stability: 3 days
Reasons for Rejection: Excessive hemolysis
Days Performed: Monday-Saturday
TAT: Samples received in the Flow Cytometry lab by 3 P.M. Mon-Sat will be reported that same day. Lab will call results upon completion if contact information is provided.
Significance: Flow Cytometry provides a more accurate and objective measurement of the number of fetal red cells present in maternal blood samples. Results from this testing allows clinicians to more accurately determine the amount of Rh Immune Globulin needed for their patients.


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