Flow Paroxysmal Nocturnal Hemoglobinuria VUMC (PNH)

Department: Immunology
Test Synonym(s): LAB928, PNH
Methodology: Flow Cytometry
Reference Range: No loss of GPI-anchored proteins similar to a normal patient pattern.
Tube Type: Yellow top (ACD-A) or green top (Heparin)
Specimen: Whole blood
Alternate Specimen: Lavender top (EDTA)
Volume: 4 mL
Minimum Volume: 1 mL
Temperature: Room temperature
Stability: Room temperature: 24 hours
Reasons for Rejection: Specimen is not peripheral blood
Days Performed: Monday - Saturday
Times Performed: Varies
TAT: 24 hours
Significance: Loss of GPI-anchored proteins CD14, CD55, and FLAER on monocytes and CD16, CD55, and FLAER on neutrophils.
Special Instructions: By consult with Hematopath - Collect BEFORE 3 pm Monday - Friday and deliver to Immunopath immediately.

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