MDL BRAF V600E DNA Mut Anly (V6A)

Department: Molecular Diagnostics
Test Synonym(s): LAB5655, V6A, BRAF, V600E
CPT Codes: 81210
Methodology: Allele Specific PCR for Detection of mutation V600E (c.1799T>A) by PCR and evaluation by capillary electrophoresis; Laboratory Developed Test
Specimen: Paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10 micron thick with accompanying before and after cut H & E slides)
Alternate Specimen: 5 unstained 10 micron slides or 10 unstained 5 micron slides (2 sections per slides)
Temperature: Room temperature
Stability: Paraffin block: indefinitely
Reasons for Rejection: Insufficient tumor in tissue; Decalcified tissue - (May or may not yield interpretable results and is dependent on the degree of fixation during processing. An alternate tissue specimen is suggested if available.)
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 10 days
Significance: DNA Analysis of Mutation V600E (allele specific PCR)
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.

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