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MDL Bone Marrow Engraftment Studies (RFL)

Department: Molecular Diagnostics
Test Synonym(s): LAB3026, Identity Testing, RFL, RFL DNA Analysis
CPT Codes: 81265 (pre-transplant, donor or identity); 81266 (additional donor); 81267 (post transplant); 81268 (sorted cells)
Methodology: Analysis of DNA Polymorphisms for identification of Host and Donor Cells Post Transplantation (Identity/Discrimination Between Samples) by fluorescent PCR and evaluated by capillary electrophoresis
Tube Type: Lavender top (EDTA)
Specimen: Blood or bone marrow, paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10 micron thick with accompanying before and after cut H & E slides); buccal swabs (5); purified DNA - 1 microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers
Volume: 1 tube
Minimum Volume: 2 microtainers; purified DNA - 100ng
Temperature: EDTA and ACD-A tubes: room temperature or refrigerated; purified DNA: refrigerate or freeze; paraffin block: room temperature; buccal swab: room temperature
Stability: EDTA and ACD-A tubes - Room temperature: 2 days, Refrigerated: 7 days; Purified DNA - indefinitely @ -70C; Paraffin block - indefinitely; buccal swab - indefinitely
Reasons for Rejection: Decalcification (paraffin embedded tissue). Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Monday - Friday
Times Performed: Batched
TAT: 7 days
Significance: Analysis of DNA for 15 independently segregating polymorphic sites and 1 gender specific marker for the identification of Recipient versus Donor Cells Post Transplantation
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.


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