MDL Brain LOH (BLH)
Department: Molecular Diagnostics
Test Synonym(s): LAB3027, Loss of Heterozygosity, BLH, BRAIN LOH (1p, 10q, 17p AND 19q)
CPT Codes: 81479
Methodology: Detection of LOH associated with Gliomas at 1p, 10q, 17p and 19q by PCR and evaluation by capillary electrophoresis (tumor and normal samples); Laboratory Developed Test
Tube Type: Lavender top (EDTA) - for control
Specimen: Control: Whole blood or normal paraffin embedded tissue and TUMOR - paraffin embedded tissue:block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10 micron thick with accompanying before and after cut H&E slides)
Alternate Specimen: 5 unstained 10 micron slides or 10 unstained 5 micron slides (2 sections per slides)
Pediatric Requirements: 2 microtainers (blood control)
Volume: 1 tube
Minimum Volume: 2 microtainers (blood control)
Temperature: EDTA : room temperature or refrigerated, Paraffin block: room temperature,
Stability: EDTA - Room temperature: 2 days Refrigerated: 7 days; Paraffin block - indefinitely
Reasons for Rejection: Decalcification (paraffin embedded tissue. Client will be notified by Molecular Diagnostics Lab if specimen is rejected.
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 14 days
Significance: Detection of LOH associated with Gliomas at 1p, 10q, 17p or 19q
Special Instructions: Referring clinician must specify which chromosomal sites analysis of LOH is requested (1p or 19q or 17p or 10q or any combination). Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.
Search Help
Search Options for the Test Directory:
1. Enter the test name in the Keyword search field.
Hint: You can also enter any part of the test name to expand the search.
(Example: you can type "west" or "sed" or "rate" to find Westergren Sedimentation Rate)
2. Enter the Vanderbilt Test Code, if known.
3. Choose one of the letter boxes to display a list of all test names and keywords starting with that letter.
If you are unable to find a test in our directory, please contact VPLS at
1-800-551-5227 or e-mail emily.k.thompson@vumc.org.