MDL FLT3-ITD (FL3)
Department: Molecular Diagnostics
Test Synonym(s): LAB3031, FL3, FLT3-ITD, FLT3 Internal Tandem Duplication
CPT Codes: 81245
Methodology: Amplified by PCR using primer pairs specific for exon 14 and 15 of the FLT3 gene; evaluated by capillary electrophoresis; Laboratory Developed Test
Tube Type: Lavender top (EDTA)
Specimen: Blood, bone marrow; purified DNA - 1 microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers
Volume: 1 tube
Minimum Volume: 2 microtainers; purified DNA - 100ng
Temperature: EDTA, ACD-A tubes: room temperature or refrigerated, purified DNA: refrigerate or freeze
Stability: EDTA and ACD-A: Room temperature: 2 days Refrigerated: 7 days; Purified DNA: indefinitely at -70C
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 10 days
Significance: Associated with Acute Myeloid Leukemia
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.
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