MDL IGH Gene Rearrangement (IGR)
Department: Molecular Diagnostics
Test Synonym(s): LAB3035, B cell gene rearrangement, IGR
CPT Codes: 81261
Methodology: PCR and evaluated by capillary electrophoresis; Laboratory Developed Test
Tube Type: Lavender top (EDTA)
Specimen: Blood, bone marrow, paraffin embedded tissue: block, 5-10 unstained slides or 5 curls (NOTE: slides and curls 10 micron thick with accompanying before and after cut H&E slides), purified DNA - 1 microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria), CSF
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen type requirements
Volume: 1 tube
Minimum Volume: 2 microtainers blood or bone marrow; please call Molecular Diagnostics Laboratory for other specimen
Temperature: EDTA and ACD-A tubes: room temperature or refrigerated; purified DNA: refrigerate or freeze; paraffin block: room temperature; CSF: refrigerate
Stability: EDTA and ACD-A tubes - Room temperature: 2 days, Refrigerated: 7 days; Purified DNA - indefinitely @ -70C; Paraffin block - indefinitely; CSF - call Molecular Diagnostics Lab at 615-343-8121
Reasons for Rejection: Decalcification (paraffin embedded tissue). Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 10 days
Significance: Detection of a clonal population of cells by PCR
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.
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