MDL JAK2 (V617F) DNA Mut Anly (JK2)
Department: Molecular Diagnostics
Test Synonym(s): LAB3036, JK2, Janus-kinase 2 gene, V617F, JAK2 (V617F) DNA Mutation Analysis
CPT Codes: 81270
Methodology: Allele Specific PCR for Detection of Mutation V617F and evaluation by capillary electrophoresis; Laboratory Developed Test
Tube Type: Lavender top (EDTA)
Specimen: Blood, bone marrow, purified DNA - 1microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers blood or bone marrow
Volume: 1 tube
Minimum Volume: 2 microtainers blood or bone marrow; purified DNA - 100ng
Temperature: EDTA and ACD-A: Room temperature or refrigerated, purified DNA: refrigerate or freeze.
Stability: EDTA and ACD-A tubes - Room temperature: 2 days Refrigerated: 7 days; Purified DNA - indefinitely @ -70C
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Once a week - variable days
Times Performed: Batched
TAT: 10 days
Significance: Associated with Myeloproliferative disorders
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
Click here to print the Molecular Diagnostics requisition.
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