MDL KIT D816V DNA Mut Anly (K17)
Department: Molecular Diagnostics
Test Synonym(s): LAB3037, K17, Kit exon 17 (D816V), N/A
Test Components: N/A
CPT Codes: 81273
Methodology: Allele specific PCR for detection of mutation D816V and evaluation by capillary electrophoresis; Laboratory Developed Test
Reference Range: N/A
Tube Type: Lavender top (EDTA)
Specimen: Blood, bone marrow, purified DNA - 1 microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A) for blood or bone marrow
Pediatric Requirements: 2 microtainers blood or bone marrow
Volume: 1 tube
Minimum Volume: 2 microtainers blood or bone marrow; purified DNA - 100ng
Temperature: EDTA, ACD-A tubes: refrigerated or room temperature; purified DNA: refrigerate or freeze
Stability: EDTA and ACD-A tubes: Room Temperature - 2 days, Refrigerated - 7 days; Purified DNA - indefinitely at -70C
Reasons for Rejection: Client will be notified by Molecular Diagnostics Lab if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: 1 time per week, variable days
Times Performed: Batched
TAT: 10 days
Significance: Associated with Acute Myeloid Leukemia
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
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