MDL MYD88 Mut (M88)
Department: Molecular Diagnostics
Test Synonym(s): LAB5710, M88
CPT Codes: 81305
Methodology: Allele Specific PCR Detection of Mutation 794T>C by PCR and evaluation by capillary electrophoresis; Laboratory Developed Test
Tube Type: Lavender top (EDTA)
Specimen: Blood, Bone Marrow, purified DNA - 1 microgram (NOTE: refer to Reasons for Rejection for DNA extraction criteria)
Alternate Specimen: Yellow top (ACD-A); 5 unstained 10 micron slides or 10 unstained 5 micron slides (2 sections per slides)
Pediatric Requirements: 2 microtainers blood
Volume: 1 tube
Minimum Volume: 2 microtainers blood or bone marrow; purified DNA - 100ng
Temperature: EDTA and ACD-A: room temperature or refrigerated; paraffin embedded tissue: room temperature; purified DNA: refrigerate or freeze
Stability: EDTA and ACD-A tubes- room temperature: 2 days; refrigerated: 7 days; purified DNA - indefinitely @-70C
Reasons for Rejection: PET: de-calcified tissue (may or may not yield interpretable results and is dependent on the degree of fixation during processing). Client will be notified by Molecular Diagnostic Laboratory if specimen is rejected. Purified DNA - externally extracted nucleic acid received for clinical testing only accepted from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by College of American Pathologists (CAP) and/ or CMS. Documentation stating that the DNA was extracted in a CLIA lab will be required and should accompany the DNA specimen. DNA samples received from facilities not meeting requirement will be rejected. The Molecular Diagnostic Laboratory medical director will contact the referral facility.
Days Performed: Once week - variable days
Times Performed: Batched
TAT: 10 days
Significance: Associated with myeloid differentiation
Special Instructions: Call or email the Molecular Diagnostics Laboratory at 615-343-8121 or moleculardiagnosticlab@vumc.org if assistance is needed for specimen submission or if the patient has received a transfusion in the prior 2 weeks or has a history of a stem cell transplant.
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