MIDL PCR HIV-1 Genotyping (HVG)
Department:
TEMPORARY SEND OUT-ARUP-CALL 615-875-5227 FOR INFO
Test Synonym(s): LAB878, HVG, HIV resistance testing, HIV1 Genotyping
CPT Codes: 87901
Methodology: PCR (Polymerase Chain Reaction) followed by nucleotide sequence analysis of amplicon
Reference Range: Resistance or resistant mutations reported
Tube Type: White top PPT (EDTA with gel) or lavender top (EDTA)
Specimen: Plasma
Alternate Specimen: Need Medical Director approval
Pediatric Requirements: 2 mL EDTA
Volume: 4 mL EDTA
Minimum Volume: 2 mL EDTA
Temperature: Room Temperature: same day, refrigerated: overnight, frozen: longer than overnight
Stability: Room temperature: same day, refrigerated: overnight, frozen: longer than overnight
Reasons for Rejection: Alternative specimen sent without Medical Director approval
Days Performed: Weekly
Times Performed: Monday
TAT: 3-10 days
Significance: Therapeutic failure for HIV infection has been closely linked with resistance to antiretroviral drugs. Mutations in the HIV-1 reverse transcriptase (RT) and protease targeted by antiretrovirals can confer drug resistance. Genotypic testing detects resistance-associated mutations in the RT- and protease-encoding genes. Resistance testing is recommended for (1) patients with acute HIV infection, (2) antiretroviral therapy (ART)-naive patients entering HIV care, (3) patients with virologic failure of ART, (4) patients with suboptimal suppression of viral load, and (5) HIV-positive pregnant women. (See Guidelines for the Use of Antiretoviral Agents in HIV-1-Infected Adults, Dept. of Health and Human Services, Oct. 14, 2011).
Special Instructions:
Click here to print the Molecular Diagnostics requisition.
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