Estradiol Lvl (E2)
Department: Chemistry
Test Synonym(s): LAB523, E2, Estradiol Level
CPT Codes: 86270
Methodology: Chemiluminescent Microparticle Immunoassay (CMIA)
Reference Range: M/F 15 days-11 mo: 0-24 pg/mL , Males - 1-10 yrs: 0-13 pg/mL; 11-14 yrs: 0-28 pg/mL;, 15-18 yrs: 0-38 pg/mL;, 19-150 yrs: 11-44 pg/mL; Females - 1-8 yrs: 0-10 pg/mL; 9-11 yrs: 0-94 pg/mL, 12-13 yrs: 11-172 pg/mL; 14-150 yrs: No reference range - see interpretive table. Normal Menstruating Females - Follicular Phase: 21-251 pg/mL; Mid-Cycle Phase: 38-649 pg/mL; Luteal Phase 21-312 pg/mL; Postmenopausal Females: 0-30 pg/mL.
The Vanderbilt Estradiol Immunoassay can accurately quantitate to 25 pg/mL. If you suspect your patient is within or below this range, consider ordering a more sensitive test (e.g., LC-MS/MS).
Tube Type: Red top tube (no gel)
Specimen: Plasma
Pediatric Requirements: 1 red top microtainer
Volume: 1 mL plasma
Minimum Volume: 1 Red top
Temperature: Refrigerated
Stability: Room temperature: 24 hours; Refrigerated: 7 days, Frozen: 2 months
Reasons for Rejection: QNS, EDTA tube, specimen not separated
Days Performed: Daily
Times Performed: Continuous
TAT: 1 day
Significance: Consider alternate method for patients on Fulvestrant therapy due to significant cross reactivity.
Special Instructions: Specimens should be centrifuged immediately. WARNING: Do NOT order on patients on fulvestrant therapy due to significant cross reactivity. Consider alternate testing method. This assay demonstrates positive interference with Mifepristone. Samples should not be collected from patients receiving Mifepristone for at least 2 weeks.
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